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| The Changes of Biological Clock Genes’ Expression in PBLC of HIE Neonates |
| Suzhou Municipal Hospital，Suzhou 215002，China |
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Abstract Objective：To study the effect of circadian rhythm disorder induced by HIE in clock genes through the comparison changes of hypoxic ischemic encephalopathy full-term neonates and normal neonates in the peripheral blood lymphocytes cell（ PBLC） in biological clock gene Clock mRNA， Bmal1 mRNA levels.Method：32 patients of HIE and 24 normal full-term newborns were determinated by semi quantitative reverse transcription polymerase chain reaction （RT-PCR） in PBLC Clock mRNA， Bmal1 mRNA，the difference between groups were compared.Result：In groups HIE the levels of Clock mRNA and Bmal1 mRNA displayed significantly lower than the control groups， the difference was statistically significant（P<0.05），the changes trend of Bmal1 mRNA and Clock mRNA in peripheral blood lymphocytes of HIE groups were basically in the same.Conclusion：The expression of Clock mRNA and Bmal1 mRNA were abnormal in PBLC of HIE group， which may plays a certain role in hypoxia and ischemia occurred after the disorder of circadian rhythm.
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