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| Expression and Clinical Significance of IL-10 and IFN-γ mRNA in Colon Cancer Tissue |
| Jinlong Community Health Service Center of Futian District People’s Hospital in Shenzhen，Shenzhen 518031，China |
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Abstract Objective：To investigate the gene expression of interleukin-10（IL-10） and interferon-γ（IFN-γ） colon tissue and methylation status of the promoter region.Method：Randomly selected from January 2011 to June 2014 colon cancer patient’s tissue 80 cases of colon cancer group， 80 cases of corresponding adjacent tissues as adjacent group，ad 100 cases of normal healthy colon tissue as the control group.Using bisulfite sequencing method（ BSP），methylation specific PCR（MSP） and real-time quantitative PCR（ RT-PCR） detection of promoter methylation of three groups of patients with colonic tissue IL-10 and IFN-γ gene and mRNA expression status. Result：Comparison of the three groups in the tissue IL-10 and IFN-γ mRNA， the differences were statistically significant（P<0.05）. Colon cancer group and adjacent group of IL-10 mRNA were higher than the control group（P<0.05），IFN-γ mRNA were lower than in the control group（P<0.05）.Three groups of IL-10 gene promoter region-110，-185 sites methylation rates，the difference was statistically significant（P<0.05）.Colon cancer group and adjacent group organization of IL-10 gene promoter region-110 were higher than the control group（P<0.05），colon cancer group IL-10 gene promoter region-185 were lower than in the control group（P<0.05）；IFN-γ gene promoter methylation of the colon cancer group>the adjacent group>the control group， the differences were statistically significant（P<0.05）. Conclusion：IL-10 and IFN-γ gene promoter methylation status affect its expression in colon cancer， colon cancer patients in TH2 polarization state， closely related to the development of colon cancer.
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