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| Modified Establishment of Wistar Rats Ischemia-Reperfusion Injury Model |
| The People’s Hospital of Qinghai Province，Xi’ning 810000，China |
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Abstract Objective：To establish a simple， reliable model of middle cerebral artery occlusion-reperfusion model in wistar rats.Method：On the basis of the method of Longa， 60 male wistar rats were randomly divided into two groups， 30 rats in each group. In the experimental group， the proximal part of the right common carotid artery（ CCA） was ligated， the distal part of the CCA was clipped， then a small cut was made in CCA， thread was put into the internal carotid artery（ ICA） through the cut of CCA to block the blood flow in the right of middle cerebral artery. One hour later，the thread was draw out under the condition of anesthetization， then the distal part of right CCA was ligated. In the control group， the proximal and distal part of the right common carotid artery（ CCA） was ligated without putting thread into ICA. After operation， brain tissue was taken out to undergo TTC staining， and neurological deficit sign scores were performed.
Result：The successful rate of modified MCAO/R model was 82.75%， the average time of operation was（ 20.4±5.3） min. In the experimental group， the right eye fission was smaller， the left extremities were paralyzed， and animals turn right while walking. The neurological deficit sign score was 3-4， the brain tissue supplied by right middle cerebral artery was pale under TTC staining， the areas of infarction was stable and the average area of infarction was about（ 34.62±5.32）%. There was no any neurological deficit in control group， and no positive results under TTC staining.Conclusion：Modified model of wistar rat ischemia-reperfusion injury has been established.
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