Abstract Objective： To study the effects of combined application of fluoride and the gallnut water extract on the growth and acidogenic metabolism of Streptococcus mutans（S.mutans）.Method：10 mL of sterile culture tube 120 were selected and divided into 12 groups， 1 group was control group，and 11 groups were experimental group，each group of 10. The experimental group standard S. mutans were cultured in vitro in the TS medium which contained 5% sucrose and different concentration of NaF（ 0.01%，0.02%） and/or gallnut water extract（ 0.25，0.5，1 mg/mL），and the medium without neither NaF nor gallnut water extract act as control group. The optical density（ OD） value of the medium and pH change before and after the culture was determined. Result： Compared with the control group， the experimental group
S.mutans growth was obviously inhibited， and with the increase of amount of sodium fluoride and gallnut water extract concentration of culture， the number of S.mutans showed a concentration dependent decrease； with the increase of sodium fluoride and the culture medium of gallnut water extract concentration， pH changes of medium of gradually reduced， NaF combined with gallnut water extract showed no obvious synergistic effect. Conclusion： NaF and gallnut water extract could significantly inhibit the growth and acidogenic metabolism of S. mutans. No obvious synergistic effect was observed while the NaF combined with gallnut water extract.
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