Abstract Objective：To investigate the effect of Hes1 on adult neurogensis from the level of neurogenesis and Hes1 expression in dentate gyrus of hippocampus between neonatal mice and adult mice.Method：The C57BL/6 male mice were separated into two groups：the newborn mice was as control for neonatal group（10 days old，N group），and the adult mice（ 4 months old， A group） were assigned to adult group，8 mice in each group.The immunofluorescence staining of Hes1，Brdu，Doublecortin（ DCX） and NeuN were performed and the cell count was corresponding recorded，double positive cells types and level of expression were preliminary analyzed of Brdu，Hes1.The hippocampal dentate gyrus was separated in microscope，Hes1 protein levels were detected by Western blot， the differences of two groups’Hes1 protein expression levels were observed and compared.Result：（1）Hes1 protein levels of A group was hingher than the
N group from Western blot detection，the difference was statistically significant（P<0.05）；（2）Hes1 positive cells mainly distributed in the dentate gyrus granular cell lower， and most Hes1 positive cells expressed Brdu at the same time；（3）the group A of Hes1（+） cells significantly was more than N group， and BrdU（+） obviously less than N group，the difference was statistically significant（P<0.05）.Conclusion：The different expression of Hes1 from neonatal mice and
adult mice in dentate gyrus of hippocampus，lead to different neurons generated levels， indicating high expression of Hes1 may inhibit neurogenesis.
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