Abstract Objective：To study the effect of siRNA-SIAH on α-synuclein autophagy and UPS degradation in SH-SY5Y.Method：After transfection of siRNA-FAM cells with fluorescent labeled SH-SY5Y， 3 siRNA were professional designed for SIAH，the protein level of SIAH expression was measured by Western Blot， one of the most efficient siRNA was selected.CCK-8 assay was used to detect the activity of siRNA-SIAH in SH-SY5Y cells. Flow cytometry was used to detect the apoptosis of siRNA-SIAH in SH-SY5Y cells.Western Blot was used to detect the protein level expression ofα-synuclein，LC3，E1，and P53.qRT-PCR was used to detect the level of mRNA in SIAH，α-synuclein，LC3 and E1. Confocal microscopy was used to detect the positioning of SIAH，α-synuclein and LC3-Ⅱ.Result： 3 siRNA cells successfully transfected SH-SY5Y，the transfection efficiency was 89%. The Western results showed that the expression of SIAH was significantly decreased in siRNA-2# cells， then studied this sequence. CCK-8 assay showed that the SH-SY5Y cell activity increased after siRNA interference， and the flow cytometry results showed that siRNA could inhibit the apoptosis of SH-SY5Y cells， which was significantly different from the control group and the negative control group（P<0.05）.With Western Blot method，the protein levels of α-synuclein， LC3 and P53 in siRNA-2# group were significantly lower and E1 was higher than that in control group and negative control group， the differences were statistically significant（P<0.05）.With qRT-PCR method，the mRNA level of SIAH，α-synuclein and LC3-Ⅱ mRNA in siRNA-2# group were significantly lower and E1 was higher than that in control group and negative control group，the differences were statistically significant（P<0.05）.Conclusion： Using siRNA to interfere with SIAH gene， SIAH gene can be used as a new target in the treatment of Parkinson’s disease by promoting the ubiquitin proteasome system to reduce SH-SY5Y in α-synuclein.
