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| The Influence of Zhiyixiao on Visfatin Protein Expression of NAFLD Rats |
| The Chinese Medicine University of Zhejiang，Hangzhou 310053，China |
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Abstract Objective：To observe the influence of Zhiyixiao on experimental non-alcoholic fatty liver disease（NAFLD） rats liver tissue pathology and visfatin protein expression and investigate the mechanism of action of Zhiyixiao in the treatment of NAFLD. Method：High fat diet were prepared for NAFLD rat model.60 rats were divided into low， medium and high Zhiyixiao of treatment group，Yishanfu capsule in the positive control group，saline control group，normal control group，10 rats in each group.The rat liver tissue homogenate TC and TG level were detected；fatty degeneration of liver cells and the liver tissue inflammatory changes were microscopically observed by HE staining in all groups；the rat liver tissue visfatin levels were detected by ELISA. Result：The levels of TC and TG in the rats’ liver homogenate of treatment group compared with the model group，the differences were statistically significant（P<0.05）； the liver cell inflammation activity was significantly improved， liver tissue visfatin protein expression was significantly decreased， the differences were statistically significant（ P<0.05），but compared with the Yishanfu group， each dose treatment group were not statistically significant differences（ P>0.05）. Conclusion：Zhiyixiao can inhibit the production of TC and TG in the liver cells，improve liver steatosis and liver tissue inflammatory diseases， improve liver tissue visfatin expression level and has similar efficacy with Yishanfu，the mechanism of NAFLD may be related to the expression of visfatin protein in liver tissue.
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