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| Protective Effect Mechanism Study of Thrombin Inhibitor Nexin-1 in Brain Edema after Intracerebral Hemorrhage in Rats |
| Panshi Hospital of Caoxian，Caoxian 274400，China |
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Abstract Objective：To explore the protective effect mechanism of thrombin inhibitor nexin-1 on brain edema after intracerebral hemorrhage.Method：90 rates were randomly divided into the control group， the model group and the intervention group.The model of cerebral hemorrhage was made by caudate nucleus injection of autologous arterial blood. In the modeling process，the intervention group was injected with thrombin inhibitor nexin-1 in the caudate nucleus，the rest group were injected with Normal Saline.12 hours， 24 hours and 36 hours after the injection，the PAR-1 in brain tissues was detected by immunohistochemistry， the Caspase-3，Claudin-5 and ZO-1 in brain tissues were detected by western blotting method.Result：The PAR-1 and Caspase-3 in brain tissues of the model group were significantly higher than those of the control group and the intervention group，the differences were statistically significant（P<0.05）. The Claudin-5 and ZO-1 in brain tissues of the model group were significantly lower than those of the control group， the Claudin-5 and ZO-1 in brain tissues of the intervention group were significantly higher than those of the model group，the differences above were all statistically significant（P<0.05）.Conclusion：The protective effect mechanism of thrombin inhibitor nexin-1 on brain edema after intracerebral hemorrhage may through reducing the expression of PAR-1，Caspase-3 and increasing the expression of Claudin-5 and ZO-1 to achieve.
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