Abstract Objective：To investigate whether FK1706 can promote the axon regeneration of peripheral nerve for rats after neural transplantation.Method：20 male SD rats were randomly assigned into two groups after they were performed with left sciatic nerve transection and autologous radial nerve grafting.Group A received FK1706（ 0.32 mg/kg） local intramuscular injected once a day on left lower limb for 8 weeks， while Group B as control were routinely fed without special intervention.Electrophysiological studies of left sciatic nerve were performed.The number and area of the regenerated myelinated nerve fiber in cross section pane， and wet muscle weight of the left gastrocnemius muscle were measured，respectively，at 8 weeks after operation.Result：The number of myelinated nerve fiber in proximal end， two groups has no significant difference in statistic（ P>0.05）， however， in distal end，number of myelinated nerve fiber in Group A was significantly higher than in Group B（P<0.01）. The thickness of myelin sheath（ mt） of the myelinated nerve fiber， and the ratio that bare axon diameter divided by the total fiber diameter（ d/D） were more favorable in Group A than the other， which difference has statistical significance（P<0.05）. The wet muscle weight of left gastrocnemius muscle， as well as the compound motor action potential（ CMAP） amplitude and motor nerve conduction velocity（ MNCV） in Group A，were much better than in Group B，with significant differences（ P<0.01）.Conclusion：FK1706 has neurotrophy effect that，
significantly promote neuroregeneration in rats after autologous nerve transplantation repairing surgery.
|
|
|
|
|
|
