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Abstract Objective： To observe the expression inhibition of miR-103-a-3p in PANC-1 pancreatic cancer cell lines infected by lentivirus with miR-103a-3p-inhibitor， and to provide theoretical basis for elucidating the pathogenesis of pancreatic cancer and screening novel target genes for the treatment of .pancreatic cancer. Method：The expression of miR-103a-3p in two pancreatic cancer cell strains（ PANC-1 and BxPC-3） with different invasiveness and the normal pancreatic ductal epithelial cell line（ H6C7） were detected by using qRT-PCR. Lentivirus vectors with miR-103a-3p-inhibitor were constructed and used to infect PANC-1 cell with high expression level of miR-103a-3p. Transfection efficiency was observed by fluorescence microscope and the expression of miR-103a-3p were detected by qRT-PCR.Result：qRT-PCR showed that the relative quantitative expressions of miR-103a-3p in PANC-1， BxPC-3 and H6C7 cell strains were （ 4.949±0.130），（ 1.417±0.120） and（ 1.010±0.151）， respectively. miR-103a-3p was expressed at highest level in PANC-1 cell. After infected by lentivirus with miR-103a-3p-inhibitor， PANC-1 cells were found to express green fluorescent proteins at high level. qRT-PCR indicated that there were no significant differences in the relative quantitative expressions of miR-103a-3p between PANC-1 cells uninfected by lentivirus with miR-103a-3pinhibitor vector（ 1.002±0.160） and PANC-1 cells infected by lentivirus with negative control vector（ 0.956±0.250），but significant reduction of the relative quantitative expressions of miR-103a-3p in PANC-1 cells infected by lentivirus with miR-103a-3p-inhibitor vector（ 0.317±0.320） were detected（P<0.05）.Conclusion：miR-103a-3p is expressed at higher level in pancreatic cancer cells with stronger invasion ability. miR-103a-3p-inhibitor can be transfected stablely into PANC-1 cells and inhibit the expression of miR-103a-3p.
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