Abstract Objective：To compare the consistency of HER-2 in breast infiltrating ductal carcinoma by IHC and FISH detection.Method：HER-2 of 118 cases with breast invasive ductal carcinoma were tested by FISH and IHC detection，and compared the results correlation.Result：118 specimens of invasive ductal carcinoma were seleced， The results tested by IHC were as follows：14 cases（-）；20 cases（1+），55 cases（2+），29 cases（ 3+）.The results tested by FISH were as follows： 53 cases >2.2， 64 cases <1.8，1 case was 1.8-2.2.HER-2 positive coincidence rate was 96.6%（28/29） by IHC detection in positive cases by FISH detection，HER-2 negative by FISH were in negative of IHC detection， the coincidence rate was 100%（34/34）.25 HER-2 positive cases by FISH detection were among 55 cases of HER-2（2+） by IHC detection， and 30 were the negative.5 cases on chromosome multi-body in 17 were found by FISH
detection，its incidence rate was 4.24%.HER-2 expression in 8 cases with of breast cancer exists heterogeneity by IHC detection，and the heterogeneity was also confirmed in FISH detected.Conclusion：HER-2 of breast infiltrating ductal carcinoma by IHC detection was high consistency with FISH detection，HER-2 cases（2+） by immunohistochemical need to be detected by FISH.
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