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| Application of Myocardial Infarction Model through Ligating of Coronary Artery under Staining Guidance in Rats |
| Guangdong Medical College Affiliated Hospital， Zhanjiang 524023， China |
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Abstract Objective：To show the anatomy of left coronary artery in rats and discuss the advantage of the death rate， success rate and stability of myocardial infarction model through ligating of the coronary artery under staining guidance.Method：8 heart harvested from sacrificed rat and irrigated coronary artery with saline injection after aortic intubation.The best concentration of agarose gel were identified， 1%， 2%， 3% and 4% agarose gel were used to perfuse aortic root at 37 ℃ .101 male rats were perfused to show the anatomy of left coronary artery， then made ligation strategies though analyzed coronary artery distribution， and compared the advantages and disadvantages in death rate，success rate and advantage of stability between high and low ligation.Result：It showed the coronary artery clearly though aortic perfusion by 2% agarose gel at 37 ℃， and the coronary anatomy can mainly be classified into 4 groups.We had made stable myocardial infarction model both in high and low ligation.Conclusion：The method of ligation of the coronary artery under staining guidance can produce stable rat myocardial infarction model both in large and middle infarct size.
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