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| Early Drug Intervention of Connective Tissue Growth Factor by Danggui Buxue Granules to Prevent Kidney Damage and Renal Vascular Lesions Induced by Hypertension |
| Henan Province Hospital of Traditional Chinese Medicine，Zhengzhou 450000，China |
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Abstract Objective：To investigate the effect of early drug intervention of Danggui Buxue granules expression of CTGF in Spontaneously Hypertensive RatS（SHR）on renal interstitial fibrosis and renal vascular lesion.Method：SHR were randomly divided into early group and late group.Early group and late group were then divided into danggui buxue granules group（DG group） and normal saline group（ NS group）.Early group was choosed 8 weeks old SHR and late group of 12 weeks old SHR. Normal group was choosed WKY rats at the same age in early and late groups. The rats were treated with DG 6 g/（kg·d）by gastric gavage in DG group and equivalent normal saline by gastric gabage in NS group and WKY rats，the rats were sacrificed at 28 weeks old.Serum creatinine，urea nitrogen and urinary β2 microglobulin were detected.Pathological changes of the renal tissue were observed by HE staining；the mRNA expression of CTGF was detected by real-time PCR， and the protein expression of CTGF was detected by Western Blot.Result：Compared with NS group， early DG group serum creatinine，urea nitrogen ，urinary β2 microglobulin ，the renal interstitial damage index，the degree of vasular lesions ，the expression of CTGF mRNA and CTGF were significantly reduced，the differences were statistically significant（P<0.05）. In late group， compared with NS group，DG group of urinary β2 microglobulin，the renal interstitial damage index， the degree of vasular lesions ，the expression of CTGF mRNA and CTGF reduced， the difference was statistically significant（P<0.01）. The effection in early group was better than late group，DG was no effect on blood pression in SHR（P>0.05）. Conclusion：Early intervention by DG can significantly attenuates renal interstitial fibrosis and renal vascular lesions in SHR by suppressing the expression of CTGF，the effection was no relationship with blood flow dynamics.
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