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Abstract Objective：To compare several extraction methods of one head mite mitochondrial genome.Method：According to reference method，the total DNA of one head mite were extracted by methods of Phenol chloroform method，Chelex-100 method and Proteinase K method.Result：Phenol chloroform method failed to amplify the purpose gene，but Chelex-100 method and Proteinase K method were successfully amplified out of the purpose gene.Conclusion：Chelex-100 method and Proteinase K method can be used in one head mite mitochondrial genes extraction，the large amount of template can be obtained with the Chelex-100 method，but its extraction efficiency is less than the Proteinase K method.
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