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| Effects of REM-PCL on the Ability of Anti-lipid Peroxidation of Canine Myocardial Mitochondrial during Ischemia/Reperfusion |
| Affiliated People’s Hospital of Guiyang Medical College，Guiyang 550002，China |
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Abstract Objective：To observe the effect of remifentanil-poly-caprolactone（REM-PCL）on the ability of antilipid peroxidation of canine myocardial mitochondrial during cardiopulmonary bypass（CPB）.Method：Twelve healthy dogs undergoing CPB were randomly allocated into control group（group C，n=6）and REM-PCL pretreatment group（group RP，n=6）.All the dogs were anesthesitized with 2.5% sodium pentobarbitone 25mg/kg.After tracheal intubation，the myocardial
ischemia-reperfusion injury model during CPB was made by declamping（DC）after aortic cross-clamping（AC）for 60 min.In group P，REM-PCL 0.2 mg/kg was administered intravenously before CPB while in group C physiological saline was given instead of REM-PCL.Myocardial mitochondria were isolated before CPB，60min after AC，30 min and 60 min after DC for determination of mitochondrial swelling degree（MSD）， malondialdehyde（MDA），glutathione peroxidase（GSH-PX），superoxide dismutase（SOD）and total anti-oxidation capacity（T-AOC）.Result：The myocardial mitochondria of MSD and MDA were increased while GSH-PX，SOD and T-AOC were decreased after AC as compared to the value before CPB in both groups（P<0.01）. MSD and MDA were increased while GSH-PX，SOD and T-AOC were decreased after DC as compared to the value before CPB in group C（P<0.01）.The myocardial mitochondria of group RP at 60min after DC had not significant differences in content of MSD，MDA，GSH-PX，SODand T-AOC（P>0.05）.MSD and MDA were distinctly higher while GSH-PX，SOD and T-AOC were distinctly lower at 60min after AC and after DC in group RP than those in group C（P<0.01）.Conclusion：Pretreatment with REM-PCL is effective in improving the antioxidation ability of myocardial mitochondria and attenuate myocardial mitochondrial injury.
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