Objective：To explore the relationship between treatment compliance and effect in patients with epilepsy and analyze the impact of factors in order to improve the treatment of epilepsy in children.Method：328 cases were collected from our hospital during January 2010 to January 2012，compliance cases were 207 and others were 121，combined with questionnaires to analyze the relation between compliance and treatment effect.In the process，we used Logistic regression to find out the relative factors of compliance.Result：The effective rate in compliance group was 97.10% and 65.29% in non-compliance group，the difference was statistically significant（ P<0.05）.Logistic regression shows adhere to medication，earlier treatment，strong awareness of clinical adherence to regular follow were the main factors affecting compliance.Conclusion：Epilepsy children’s treatment effect has relation with compliance，according to earlier treatment，improve treatment sense，regular follow can do a positive effect in epilepsy children.

2015 Vol. 12　(17):　3-5 [Abstract]（ 606 ） HTML (1 KB)  PDF  (714 KB)  ( 339 )

Objective：To investigate the family early intervention of brain injury in high-risk infants with hospital guidance individual following vojta posture reflex the five positive.Method：120 cases randomly divided into three groups，group A for 40 cases，group B for 40 cases，group C for 40 cases.The group A patients received individual instruction home early intervention treatment； group B patients received hospital early intervention treatment； group C patients received general family early intervention for non-individualized instruction.The patient were observed for six months，and the effect of the three groups were compared.Result：After six months of therapy treatment，，the comparative efficacy reveal had no statistical difference in group A and group B，while compared with C group，there was notable statistical difference（P<0.05）. Conclusion：The hospital guidance individual instruction of individual home early intervention can effectively improve the overall development level of high-risk infants following 5 positive brain injury.

2015 Vol. 12　(17):　9-11 [Abstract]（ 652 ） HTML (1 KB)  PDF  (706 KB)  ( 274 )

Objective：To observe the cancer stem cell like characteristics of CD133⁺ and CD133^- in colon cancer cell line SW480，and to analyze the differences of expressions of Bmi-1 gene in CD133⁺ and CD133^- cells.Method：CD133⁺ and CD133^- cells were sorted by flow cytometry，the abilities of sphere-forming and cell proliferation of CD133⁺ and CD133^- cells in vitro were observed.The expressions of Bmi-1 gene in CD133⁺ and CD133^- cells were detected by Real-time PCR.Result：（1）CD133⁺ cells accounted for 7.02% in SW480.（2）CD133⁺ cells were formed spheres within 4 culture days and grow fast in vitro.CD133^- cells can not formed spheres，but can adhered and differentiated in medium with serum.In serum-free medium，the abilities of proliferation in CD133⁺ cells were bigger than those in CD133 cells.（3）The expressions of Bmi-1 mRNA in CD133⁺ cells were significantly higher than those of CD133^- cells.Conclusion：The CD133⁺ cells account for a little proportion in SW480，but have the higher abilities of proliferation.Bmi-1 mRNA express highly in CD133⁺ cells，may be related to the carcinogenesis.

2015 Vol. 12　(17):　15-18 [Abstract]（ 674 ） HTML (1 KB)  PDF  (748 KB)  ( 363 )

Objective： To investigate and analyze the clinical treatment effect of diabetes mellitus complicated with severe oral and maxillofacial space infection. Method： 31 patients with diabetes mellitus of severe oral and maxillofacial space infection were treated in our hospital， the treatment effect and laboratory results were summarized. Result： 31 patients， 1 cases died of heart failure，30 cases were cured and all of them regular expand abscess incision drainage，tracheotomy of 6，3 cases with mediastinal abscess， 1 case of cerebral infarction. Conclusion： The key of diagnosis and treatment of diabetic patients with oral and maxillofacial space infection is recognized early， control of blood glucose and maintain airway patency， timely incision drainage， appropriate use of antibiotics， support therapy and control of complications and accompanying diseases are very important to improve the cure rate.

2015 Vol. 12　(17):　21-23 [Abstract]（ 656 ） HTML (1 KB)  PDF  (694 KB)  ( 420 )

Objective：To assess the value of 320 slice volume CT venography（ CTV） in diagnosis of lower extremity deep venous thrombosis（DVT）.Method：The CTV data of 49 patients with DVT confirmed by clinical data were retrospectively studied and compared　with DSA and US.Result：46 cases with DVT were detected by CTV，including acute thrombosis 26 cases and old thrombus 20 cases.When compared with DSA and Doppler ultrasound，the sensitivity of CTV in detecting acute thrombosis was 100% and in detecting old thrombus was 93.8%. Conclusion：320 slice volume CTV has significant value in the diagnosis of lower extremity deep venous thrombosis.

2015 Vol. 12　(17):　25-27 [Abstract]（ 491 ） HTML (1 KB)  PDF  (719 KB)  ( 538 )

Objective：To analyze and discuss the effect of PCI after thrombolysis and direct PCI on patients with acute myocardial infarction.Method：70 patients with acute myocardial infarction were selected from January 2012 to December 2014 in cardiovascular department of internal medicine of our hospital as the research object the clinical test，their clinical data were retrospectively analyzed.Among them，31 patients were treated thrombolytic therapy after percutaneous coronary intervention as the after thrombolysis PCI group，the other 39 patients were treated primary percutaneous coronary intervention as the direct PCI group，clinical effect and changes of left ventricular contractile ability as well as complications of two groups after treatment were observed and compared. Result：After clinical treatment，the 70 patients’ condition were greatly improved，after thrombolysis PCI group ’s clinical effective rate was 70.97%，direct PCI group was 97.44%，the difference was statistically significant（P<0.05）； two groups patients’ left ventricular end-diastolic diameter，left ventricular end-diastolic volume index and left ventricular ejection fraction in 20 days and 80 days after treatment，were obviously improved than after 3 days treatment，direct PCI group was better than after thrombolysis PCI group，the difference was statistically significant（P<0.05）； after discharge，visiting survey results suggested that recurrence and complications such as heart failure，the direct PCI group incidence was 10.26%，obviously lower than after thrombolysis PCI group 35.48%，the difference was statistically significant（P<0.05）. Conclusion：Percutaneous coronary artery interventional therapy has a good therapeutic effect on patients with acute myocardial infarction，in contrast，direct PCI treatment of comprehensive effect is superior to after thrombolysis treatment.

2015 Vol. 12　(17):　30-32 [Abstract]（ 574 ） HTML (1 KB)  PDF  (707 KB)  ( 369 )

Objective：To explore the changes and clinical significance of serum transferrin and C reactive protein in neonatal jaundice.Method：100 cases of neonatal jaundice were selected from March 2013 to March 2014 in our hospital and were grouped according to the causes and total bilirubin levels of neonatal jaundice.50 full-term and no jaundice newborns were selected as control group during the same time period.The serum TRF and CRP levels of children in different group were completed.Result：The serum TRF and CRP levels of infection factors group，perinatal factors group and other factors group compared with the control group，the differences were statistically significant（P<0.05）.But there was no significant difference in the serum TRF levels among the three groups（P>0.05）. C reactive protein level of infection factors group was significantly higher than perinatal factors group and other factors group，the differences were statistically significant（P<0.05）.The serum TRF and CRP levels of mild jaundice group，moderate jaundice group and severe jaundice group compared with the control group，the differences were statistically significant（P<0.05）.But there was no significant difference in the serum TRF level among the three groups（P>0.05）.C reactive protein level of severe jaundice group was significantly higher than mild，moderate jaundice group，the differences were statistically significant（P<0.05）.Conclusion：Serum TRF and CRP levels have certain clinical value in differentiating etiology and disease of neonatal jaundice.

2015 Vol. 12　(17):　36-38 [Abstract]（ 642 ） HTML (1 KB)  PDF  (696 KB)  ( 571 )

Objective：To study and explore the clinical efficacy of continuous renal replacement treatment（ CRRT） on acute decompensated heart failure（ ADHF） of diuretic resistance.Method：Fifty patients with acute decompensated heart failure of diuretic resistance who were admitted by the hospital from January 30 2012 to June 30 2014 were selected as research subjects，they were divided into the control group（ 26 cases，other conventional treatment of heart failure），early CRRT treatment group（ 17 cases） and conventional CRRT treatment group（ 13 cases）according to different treatment methods and treatment opportunities，the treatment efficacies of patients in the three groups were compared.Result：The differences in mutual comparisons in the breathing rate，heart rate，blood pressure，blood oxygen saturation，creatinine drop，increase scope of urine and falling range of brain natriuretic peptide were statistically significant（P<0.05）.Conclusion：ADHF applied on acute decompensated heart failure with diuretic resistance can maintain clinical indexes of patients within normal range，accelerate the decrease of creatinine，the increase of urine volume and the decrease of brain natriuretic peptide，which can promote therapeutic effects.The effect of early CRRT is more obvious，being worthy of wide application in clinical practices.

2015 Vol. 12　(17):　41-43 [Abstract]（ 640 ） HTML (1 KB)  PDF  (692 KB)  ( 478 )

Objective：To summarize the MRI features of enlarged perivascular spaces for improvement of diagnostic rate. Method：90 EPVS patients with MRI indication were selected randomly，their MRI features in respect of shape，size，position，boundary，signal and space occupying effect analyzed. Result：Multiple EPVS were visible bilaterally for all the 90 cases and there had no remarkable difference between the right and left side.The EPVS feature round，oval，linear or tubular shapes with clear boundary and the same direction as the perforator vessels. Their signals were the same as those of the cerebrospinal fluid on T1WI，T2WI and FLAIR sequences（ low signal for T1WI，high signal for T2WI and low signal for FLAIR） without the enhancement effect of contrast agent and the space occupying effect.The EPVS occur at the basal ganglia region，the centrum semiovale and subcortex，the pontine，the cerebellum，the thalamencephalon and the periventricle，and their amount increases along with the age.Rank 1 was common in EPVS rating and no obvious change was found in the follow-up cases. Conclusion：The EPVS amount increases along with the age，they mainly occur at the basal ganglia region as well as the centrum semiovale and subcortex in round，oval，linear or tubular shape with clear boundary，and their signals are the same as those of the cerebrospinal fluid on MRI sequences.

2015 Vol. 12　(17):　47-49 [Abstract]（ 833 ） HTML (1 KB)  PDF  (741 KB)  ( 507 )

Objective：To discuss the prevention effect of Sufentanil on the early hurt after the Remifentanil anesthesia. Method：84 patients who were conducted the abdominal hysterectomy treatment were selected in our hospital from March 2012 to March 2014 as the research objects，and were randomly divided into the control group and the observation group，42 cases in each group.In the observation group，they were treated with the Sufentanil to prevent the early hurt after the Remifentanil anesthesia，while in the control group，they were treated with the Fentanyl to prevent the early hurt after the Remifentanil anesthesia.Then，the hurt situations after surgeries of two groups of patients were analyzed.Result：The consciousness recovery time，extubation time and the independent breathe recovery time of two groups had no statistically significant difference（P>0.05）. The occurrence rate of the adverse reactions in the observation group was 4.76%，which was evidently lower than the control group of 21.43%，the difference was the statistically significant（P<0.05）.The oral pain score after the extubation and the VAS score in different periods after surgeries in the observation group were evidently lower than that in the control group，the difference were statistically significant（P<0.05）.Conclusion：Sufentanil has higher safety，the analgesia effect is evidently higher than Fentanyl.There is no evident respiratory depression and can decrease the occurrence of the adverse reactions.It can effectively prevent the early hurt after the Remifentanil anesthesia. Thus，it should be promoted in the clinic applications.

2015 Vol. 12　(17):　53-55 [Abstract]（ 531 ） HTML (1 KB)  PDF  (692 KB)  ( 326 )

Objective：To explore the fine application effect of perioperative patients care health education.Method：212 patients were selected in the author’s hospital according to the admission time and divided into the observation group of 110 cases and control group of 102 cases.The observation group was treated the fine nursing health education，the control group was given conventional method.Result：Compared with the control group，the observation group patients for operation related knowledge，doctor’s advice behavior and nursing work satisfaction rates were higher than that of the control group ； the nursing adverse events of observation group were significantly lower than the control group，the differences were statistically significant（ P<0.05）. Conclusion：In the fine application perioperative period patients with nursing health education，improve the effect of health education，reduce the incidence of nursing adverse events，satisfaction of patients and their families increased obviously，it is worthy of extensive application of modern health education model.

2015 Vol. 12　(17):　59-62 [Abstract]（ 602 ） HTML (1 KB)  PDF  (727 KB)  ( 412 )

Objective：To explore the influence of motivational interviewing on self-efficacy and self-management in patients with diabetes.Method：100 cases with diabetes in our hospital from November 2012 to February 2013 were selected and randomly divided into observation group and control group with 50 cases in each group.All patients were treated with basic drug therapy，in the control group patients were treated with routine care intervention and health education while the observation group plus comprehensive nursing interventions which included health education based on motivational interviews.Self-management and self-efficacy scores，glycemic control and quality of life after 6 months were compared.Result：Self-efficacy and selfmanagement of the two groups were significantly improved，with an average score of the observation group higher than that of the control group（ P<0.05）.Fasting plasma glucose and glycated hemoglobin in the observation group and the control group were significantly decreased，the mean fasting plasma glucose of the observation group was lower（ P<0.05），the difference in glycated hemoglobin between the two groups was not statistically significant（ P>0.05）.The quality of life of the observation group was significantly improved，and the control group only advanced in treatment outcome，The scores of the observation group were higher than that of the control group in all dimensions（P<0.05）. Conclusion：Motivational interviewing which guides health education has significant improvement for diabetes’ self-efficacy and self-management，it helps control blood sugar and improve the patients’ quality of life.

2015 Vol. 12　(17):　65-67 [Abstract]（ 567 ） HTML (1 KB)  PDF  (706 KB)  ( 344 )

Objective：To study the importance of positive nursing in the nursing path of Alzheimer’s disease. Method：88 patients over 60 years old who were hospitalized for treatment of Alzheimer’s disease from May 2013 to May 2014 were chosen.They were divided into group A and group B at random.Patients in the group A were given the positive nursing path with emotion while patients in the group A were cured with the application of traditional nursing path.The total effective rate，Mini-mental State Examination（ MMSE） and Activities of Daily Living（ ADL） of the two groups were compared. Result：The effective rate of group A was 75.0% and that of group B was 56.8%.MMSE and ADL of the patients in the group A were obviously better than that in the group B（P<0.05）. Conclusion：Positive nursing is important in the nursing path of Alzheimer’s disease.

2015 Vol. 12　(17):　71-74 [Abstract]（ 632 ） HTML (1 KB)  PDF  (726 KB)  ( 300 )

Objective：To study and research on the comprehensive nursing measures of outcome in patients with gestational hypertension syndrome and pregnancy outcome，provide necessary basis for clinical nursing plan selection. Method：100 cases of PIH women from January 2011 to January 2014 in our hospital were selected，under the condition of the patients were informed consent in a randomized，control group 50 cases with normal obstetric nursing，the observation group had 50 cases on the basis of conventional nursing care，comprehensive care of PIH，after the birth of two groups of patients with the disease outcome and pregnancy outcomes were compared.Result：From the point of outcome comparison，observation group of patients outcomes rate was 94.0%，control group patients was 78.0%，the observation group was significantly better than the control group，the difference was statistically significant（ P<0.05）.From pregnancy outcomes comparison，observation group average birth weight of（ 3.3±0.8）kg，Apgar score was（ 9.6±0.3）points，dystocia rate was 36.0%，control group were （2.6±0.6）kg，（8.2±0.6）and 62.0%，the observation group were significantly better than the control group，the differences were statistically significant（ P<0.05）.Conclusion：Comprehensive nursing measures for patients with gestational hypertension syndrome can effectively promote the patient outcomes，improve the pregnancy outcome，is worth in clinical promotion and application.

2015 Vol. 12　(17):　77-79 [Abstract]（ 624 ） HTML (1 KB)  PDF  (693 KB)  ( 717 )

Objective：To explore and study the nutritional care at the end of the application effect of end-stage renal disease with hemodialysis patients，to provide the necessary basis for the selection of clinical nursing plan. Method：100 patients of maintenance hemodialysis for end-stage renal disease were selected in our hospital from August 2012 to August 2014 and were randomly divided into two groups，50 cases in each group.The control group was treated with routine nursing care，the observation group was trested on the basis of routine nursing with nutritional care，the nutritional status and life the quality of two groups’ patients before and after intervention were compared. Result：Compared two groups’ patients of nutritional status before the intervention，the difference was no statistically significant（P>0.05）.After intervention，the observation group of BMI，albumin，creatinine，and hemoglobin levels were significantly higher than those in the control group，the malnutrition inflammation score（ MIS） was significantly lower than that of the control group，the differences were statistically significant（P<0.05）. Before the intervention，compared two groups’ patients quality of life scale（ SF-36） scores，the difference was no statistically significant（P>0.05）.After intervention，the observation group of general health，physiological function，physiological function，body pain，energy，social function，emotional function，physical health and mental health overall evaluation were significantly better than the control group，the difference was statistically significant（P<0.05）. Conclusion：The treatment of maintenance hemodialysis patients with end-stage renal disease is often poor nutritional status，the implementation of nutrition care reasonable nutrition level and quality of life improved significantly in patients，it is worthy of clinical popularization and application.

2015 Vol. 12　(17):　82-84 [Abstract]（ 492 ） HTML (1 KB)  PDF  (705 KB)  ( 236 )

Objective：To investigate the clinical effect of family-centered community nursing intervention on the prevention and treatment of pneumonia in children.Method：100 children with pneumonia in the community from July 2012 to June 2014 were selected as the research object.They were randomly divided into the study group and the control group，the study group was treated with community nursing intervention while the control group was treated with general nursing method. Result：After the clinical treatment，the clinical therapeutic effect of the study group was significantly better than that of the control group，the difference had statistical significance（P<0.05）. Conclusion：The effect of family-centered community nursing intervention used on the prevention and cure of children pneumonia is remarkable.It can not only effectively relieve the clinical symptoms of the patients，but also reduce the occurrence rate of children pneumonia.It is a nursing program with high feasibility，safety and effectiveness.

2015 Vol. 12　(17):　87-89 [Abstract]（ 441 ） HTML (1 KB)  PDF  (694 KB)  ( 219 )

Objective：To investigate the effect of acupuncture combined with clinical treatment of chronic pelvic inflammatory disease. Method：90 patients with chronic pelvic inflammatory disease were selected from January 2012 to February 2014 in our hospital，they were randomly divided into two groups，45 patients in each group，the control group were given pure traditional Chinese medicine treatment，the observation group were observed in the use of traditional Chinese medicine，based on the method of treatment plus acupuncture，the clinical outcomes in the two groups were compare. Result：The clinical effective rate of the observation group was 97.8%，the recurrence rate was 6.7%，in the control group were 82.2%，22.2%，the differences were statistically significant（ P<0.05）. Conclusion：Chinese herbs combined with acupuncture treatment of chronic pelvic inflammatory effect is remarkable，good clinical results，recurrence rate，help to improve the quality of life of patients，the kind of treatment should be promoted in clinical practice.

2015 Vol. 12　(17):　92-94 [Abstract]（ 635 ） HTML (1 KB)  PDF  (694 KB)  ( 200 )

Objective：To explore TCM auxiliary treatment effect on the therapeutic effect of pulmonary tuberculosis patients with sputum smear positive.Method：100 patients of pulmonary tuberculosis were selected from January 2011 to December 2013 and were divided into two groups according to randomly method，50 cases in each group.Two group patients were given medicine standard chemotherapy，the observation group was given the TCM auxiliary treatment.The curative effects after 1，3 and 6 months were evaluated，focuse on clinical symptom improvement rate，the sputum negative conversion rate and the improvement rate of image were emphatically observed.Result：After 1 months of treatment，two groups of patients with clinical symptoms improvement rate，the sputum negative conversion rate and image to learn conversion rate the differences had no statistically significant（P>0.05）.After 3 months and 6 months treatment，two groups clinical symptom improvement rate，the sputum negative conversion rate and image to learn conversion rate were observed，significantly higher than the those of the control group，the differences were statistically significant（P<0.05）.In 100 cases，7 cases occurred with abnormal liver function in the treatment process，the observation group had 3 cases，4 cases in the control group，the difference was not statistically significant（P<0.05）.Conclusion：Chinese medicine treatment of pulmonary tuberculosis patients with sputum smear positive，can play a satisfactory effect.

2015 Vol. 12　(17):　97-99 [Abstract]（ 565 ） HTML (1 KB)  PDF  (694 KB)  ( 408 )

Objective：To investigate the clinical effects of very low birth weight children given the gravity-fed and nonnutritive sucking and nursing interventions for newborn massage.Method：From January 2013 to January 2014 period，our hospital neonatal treated 62 cases of very low birth weight children were randomly divided into two groups，31 patients were given conventional treatment intermittent gastronomic feeding（ conventional group），The remaining 31 patients were given the gravityfed，non-nutritive sucking，touching nursing intervention therapy（ study group），compared to its clinical effects.Result：The group of children with a gastronomic tube indwelling time was（ 11.30±6.83） d，recovery time of birth weight（ 7.64±3.55） d，a sufficient amount of entrant feeding time（ 11.62±6.45） d，length of hospital stay for（ 24.42±11.05） d.Gastronomic tube indwelling time children in the control group was（ 15.72±7.79） d，recovery time of birth weight（ 12.45±3.80） d，a sufficient amount of entrant feeding time（ 15.41±6.52） d，hospitalization time（ 35.68±17.82） d. Indicators of childminders time in the observation group were significantly shorter than children in the control group，the difference was statistically significant（ P<0.05）.There were 5 children in the observation group（ 16.13%） in children with vomiting cases，6 patients（ 19.35%） were abdominal distension，4 patients（ 12.90%） were gastric residual，2 children（ 6.45%） with respiratory cases suspended.There were 16 children（ 51.61%） in the control group and vomiting in children cases，15 patients（ 48.39%） were abdominal distension，14 infants（ 45.16%） gastric residual，11 cases（ 35.48%） of children with breathing pauses.Feeding children was not tolerated in the observation group was significantly lighter than the control group of patients，the difference was statistically significant（ P<0.05）. Conclusion：Very low birth weight children given the gravity-fed and non-nutritive sucking and massage combined with newborn care interventions can effectively alleviate the situation of feeding intolerance，thereby improving the prognosis，protect the healthy growth of the patient.

2015 Vol. 12　(17):　105-107 [Abstract]（ 644 ） HTML (1 KB)  PDF  (694 KB)  ( 262 )

Encephalopathy rehabilitation center is a larger clinical department of Tai’an Traditional Chinese Medicine Hospital which had 48 beds independent and reached to 60 beds in fact .There are 36 staffs in the center containing doctors and rehabilitative therapists.There are more than 540 cases of in-patients every year and the treatment of outpatient service and collaboration among departments reached to almost 9000 cases each year.The key performance indicators increased year by year in the center and it is better than most of other department in our hospital.The center has not only been involved in the construction process of“ encephalopathy center” in“ eleven five”period proposed by the State Administration of Chinese Medicine，but also participated in creating the three level first-class hospital.In 2007，the center became the highlights of "Tai’an station" in the activities of "The Road of Traditional Chinese Medicine in China"，the characteristics of traditional Chinese medicine in the center was approved by the leaders of the national，provincial and city department of traditional Chinese medicine.In the development process，the department has became standard and mature gradually and became the“rehabilitation center combined traditional Chinese and western medicine of Tai’an for disabled persons”and“ traditional Chinese medicine subject”，our department has became one of the better rehabilitation departments in Shandong province.The aim of this subject is to introduce the development process and management experiences of this center for reference.

2015 Vol. 12　(17):　110-113 [Abstract]（ 579 ） HTML (1 KB)  PDF  (731 KB)  ( 418 )

Objective：To evaluate the impact of medicines policy on rational use of drugs by comparing the essential drugs and antibiotics.Method：A total of prescriptions in outpatients and emergency departments of hospital records，2011 was selected as a policy implementation group，2012 as part of the implementation of phase 1 group，2013 as part of the implementation of phase 2 group，before and after the implementation of the control study，the effectiveness and feasibility of the implementation of the policy were investigated.Result：The usage of prescriptions and essential drugs was 51% and 33% respectively.The implementation of phase 2 the intensity of use of antimicrobial agents for 38.67%，compared with the former of 73.06% were decreased significantly，the difference between the two groups had statistically significance（P<0.05）.Type I incision surgery to prevent use of antibacterial drugs proportion had decreased significantly，the difference had statistically significance（P<0.01）. Conclusion：Medicines policy and intervention measures have some effect on the promotion of rational drug use.

2015 Vol. 12　(17):　116-118 [Abstract]（ 444 ） HTML (1 KB)  PDF  (693 KB)  ( 351 )

Objective：To investigate the application of enzyme linked immunosorbent assay and immune colloidal gold technique in the detection of Chlamydia trachomatis.Method：90 patients with Chlamydia trachomatis infection from January 2012 to January 2014 in our hospital were suspected as the object，and all patients were treated by enzyme linked immunosorbent assay，immune colloidal gold technique and cell infection for detection of Chlamydia trachomatis culture，and ELISA results determination and immune colloidal gold technique in cell culture was compared，and the sensitivity was used as the gold standard to calculate two kinds of methods and specificity. Result：Enzyme linked immunosorbent assay and immune colloidal gold technique had no significant difference in positive rate of detection of Chlamydia trachomatis in female patients，and there was no statistical significance（ P>0.05），and the positive rate of male patients in the detection of Chlamydia trachomatis had significant difference（ P<0.05）； and there was no significant differences in two methods in the positive rate of Chlamydia trachomatis detection sensitivity and specificity，and there was no statistical significance（ P>0.05）. Conclusion：The method of enzymelinked immunosorbent assay and immune colloidal gold technique for detection of Chlamydia trachomatis are a very effective method，but the former for positive rate of the men of Chlamydia trachomatis is obviously higher than the latter，therefore should be based on the actual situation，flexible application.

2015 Vol. 12　(17):　122-124 [Abstract]（ 566 ） HTML (1 KB)  PDF  (693 KB)  ( 403 )

Objective：To compare the clinical curative effect of open tension-free hernia repair and laparoscopic totally extraperitoneal（ TEP） in treatment of elderly inguinal hernia.Method：66 elderly patients with inguinal hernia from May 2010 to May 2013 year in our hospital were selected，they were randomly divided into observation group and control group，33 cases of observation group were treated with laparoscopic hernia repair，33 cases of the control group use open tension-free hernia repair，amount of bleeding during the operation time，two groups of patients recorded intraoperative，postoperative pain duration record of two groups，time，cost of hospitalization，complications and inguinal hernia recurrence rate in hospital，all the test data were input statistical software for analysis.Result：The intraoperative bleeding volume in observation group was significantly lower than that of the control group，compared with significant difference（ P<0.05），but the observation group compared with the control group，operation time was slightly longer，there are differences statistically significant（ P<0.05）； postoperative pain，hospitalization time of observation group were lower than the control group，there were differences statistical significance （P<0.05），the observation group hospitalization cost was higher than the control group，compared the difference was statistically significant（ t=123.3502，P=0.0000）； the two groups the incidence of complications compared with statistical difference（ P<0.05），compared with no much difference recurrence rate.Conclusion：Laparoscopic totally extraperitoneal（ TEP） has less bleeding，hospitalization time is short，the advantages of minor aches and pains，significantly better than open tension-free hernia repair，it is worth to be popularized in clinic.

2015 Vol. 12　(17):　127-129 [Abstract]（ 633 ） HTML (1 KB)  PDF  (692 KB)  ( 386 )

Objective：To investigate the clinical treatment and prognosis of mother and child for premature rupture of membranes.Method：188 cases of obstetric　pregnant women in our hospital from March 2013 to May 2013 were selected and divided into two groups，among them 104 cases without premature rupture of membranes were selected as control group，the other 84 cases with premature rupture of membranes were selected as the observation group.Routine vaginal examination was given to the two groups of pregnant women，the character of amniotic fluid was observed，according to different circumstances induced labor and infection prevention were given to the two groups of pregnant women at a appropriate time，at the same time the condition of delivery，prognosis of maternal and neonatal were observed and compared between the two groups.Result：The conditions of delivery and prognosis between the two groups were compared. The rate of dystocia，asphyxia neonatorum，fetal distress and puerperal infection in the observation group were higher than that of the control group. The differences were statistically significant（P<0.05）. Conclusion：The pregnant women with premature rupture of membranes have higher risk of dystocia and neonatal asphyxia，it needs to strengthen monitoring in clinical practice，so as to reduce maternal injury.

2015 Vol. 12　(17):　132-135 [Abstract]（ 528 ） HTML (1 KB)  PDF  (716 KB)  ( 274 )

Objective：To approach the treatment result of subclinical hypothyroidism patients in gestation period by thyroid hormones.Method：34 patients clinical data of subclinical hypothyroidism in gestation period were selected in our hospital from August 2012 to August 2014，and were divided into two groups，no treatment group of 14 cases and treatment group of 20 cases.Result：The TSH，TG，total cholesterol abnormalities of treatment group were better than no treatment group，FT3，FT4 level were higher than no treatment group，the premature delivery，pregnancy-induced hypertension，preeclampsia，abruptio placentae and incidence of anemia during pregnancy of treatment group were lower than no treatment group，the differences were statistically significant（P<0.05）.Conclusion：The pregnancy outcome of subclinical hypothyroidism patients in gestation period by thyroid hormones are improved，the pregnancy complication is decreased，so it is worth to promote in clinical.

2015 Vol. 12　(17):　137-139 [Abstract]（ 478 ） HTML (1 KB)  PDF  (691 KB)  ( 270 )

Objective：To detect the purulent meningitis（ PM） lactic acid in cerebrospinal fluid in children with LA，interleukin-8（ IL-8） concentration changes，explore their changes in purulent meningitis and its clinical significance. Method：60 cases of purulent meningitis as the PM group，42 cases with viral encephalitis as the VE group，54 cases with no central nervous system diseases as the control group，all of children acute and recovery phase of lactic acid in cerebrospinal fluid（ LA） and IL-8 were selected and detected the change by using COBAS INTEGRA 400PLUS automatic biochemical analyzer and double antibody sand wich enzyme linked immunosorbent assay（ ELISA）.The correlation between LA and IL-8 were observed with neutrophils. Result：In PM group，LA and IL-8 in cerebrospinal fluid were（ 4.71±2.43）mmol/L and（ 261.02±58.14）ng/L higher than that in VE group（ 2.10±0.44）mmol/L and（ 94.86±41.27）ng/L，control group（ 1.95±0.51）mmol/L and（ 84.65±19.7）ng/L，the differences were statistically significant（ P<0.01）；recovery PM group LA and IL-8 were（ 3.23±2.11） mmol/L and （204.24±48.53）ng/L decreased significantly which compared with acute period，the differences were statistically significant （P<0.01）.Analysis of cerebrospinal fluid LA and IL-8 levels and neutrophil associated display：PM group LA was positively related to IL-8（r=0.482，P<0.01）； PM group IL-8 was positively associated with neutrophil（r=0.397，P<0.01）； there was no significant correlation in VE group between IL-8 and neutrophils（r=0.299，P>0.05）. Conclusion：Lactic acid and IL-8 participates in the local inflammatory response of purulent meningitis.Suggest a reference index for detection of IL-8 and LA in the cerebrospinal fluid as purulent meningitis diagnosis and illness change judgment.

2015 Vol. 12　(17):　144-146 [Abstract]（ 555 ） HTML (1 KB)  PDF  (696 KB)  ( 410 )

Objective：To explore the clinical efficacy of laparoscopic surgery for the treatment of gynecological malignancies.Method：A total of 48 patients with malignant tumors including 23 cases with endometrial cancer，1 case with uterine sarcoma，19 cases with cervical cancer，1 case with residual cervical cancer，2 cases with ovarian cancer，1 case with recurrent ovarian cancer and 1 case with synchronous primary cancers of the endometrium and ovary in our hospital from January 2011 to July 2014 underwent laparoscopic surgery.The laparoscopic surgery treatment effect was observed.Result：All the 48 cases were successfully treated by laparoscopic surgery，no one turned to undergo laparotomy.The average operation time was（ 249.29±71.83）min.The average intraoperative hemorrhage volume was（ 237.50±132.95）mL.The average pelvic lymph node resected and abdominal aorta lymph node resected were（18.61±3.06） and（ 4.07±1.42）.2 patients complicated with external iliac vein injury.1 patient complicated with bladder injury.Follow up checkups for 3-45 months postoperatively in 48 patients showed 1 case of recurrence at 9 postoperative month（stage Ⅱa cervical adenosquamous carcinoma and positive pelvic lymph node，withdrew from the treatment and died 6 months later）.Conclusion：Laparoscopic surgery for the treatment of gynecological malignancies is safe and feasible，and has satisfactory result，but the surgeon must have skilled operation technique and treatment experience of operation complication.

2015 Vol. 12　(17):　150-153 [Abstract]（ 604 ） HTML (1 KB)  PDF  (725 KB)  ( 427 )